Journal: Nucleic Acids Research

Article Title: Characterization of the Type III restriction endonuclease PstII from Providencia stuartii

doi: 10.1093/nar/gki787

Figure Lengend Snippet: The Type III enzymes EcoP15I and PstII cannot mutually activate cleavage of T7 coliphage DNA. ( A ) Representative schematic (not to scale) of the relative orientation of EcoP15I and PstII sites in lambda (λ) and T7 phage genomic DNA. Site orientations (arrowheads) are defined as in . ( B ) Cleavage of λ and T7 genomic DNA by mixtures of Type III enzymes. 500 ng of λ or T7 phage DNA was mixed with 50 nM EcoP15I and/or 129 nM PstII mixture as shown in the presence of 4 mM ATP. Where indicated AdoMet was added to 100 μM. Following incubation for 1 h at 37°C, substrate and products were separated by agarose gel electrophoresis.

Article Snippet: Cell-extracts were prepared, serially diluted, incubated for 1 h at 37°C with lambda phage DNA in NEB 3 [50 mM Tris–HCl, 10 mM MgCl 2 , 100 mM NaCl, 1 mM DTT (pH 7.9)] and DNA products separated by agarose gel electrophoresis.

Techniques: Incubation, Agarose Gel Electrophoresis

Journal:

Article Title: Functional analysis of putative restriction-modification system genes in the Helicobacter pylori J99 genome

doi:

Figure Lengend Snippet: Agarose gel (1%) showing the electrophoresis patterns of bacteriophage λ DNA digested by different amounts of restriction endonuclease, Hpy99II, encoded by JHP46. Lane 1, bacteriophage λ DNA + HindIII, φX174 + HaeIII, size standard; lane 2, λ DNA digested with 9 µl Hpy99I; lane 3, λ DNA digested with 3 µl Hpy99I; lane 4, λ DNA digested with 1 µl Hpy99I; lane 5, λ DNA digested with 0.3 µl Hpy99I.

Article Snippet: Extracts were incubated with bacteriophage λ DNA, phage T7 DNA, and adenovirus-2 DNA in NEBuffers 2 and 4 (New England Biolabs) at 37°C for 15 min and the digestion products were analyzed by agarose gel electrophoresis.

Techniques: Agarose Gel Electrophoresis, Electrophoresis

Journal:

Article Title: Functional analysis of putative restriction-modification system genes in the Helicobacter pylori J99 genome

doi:

Figure Lengend Snippet: Agarose gel (1%) showing the electrophoresis patterns of λ DNA digested by restriction endonuclease Hpy99III, encoded by JHP1049, from consecutive fractions of a heparin column. Lane 1, λ DNA + HindIII, φX174 + HaeIII, size standard; lanes 2–12, λ DNA digested with 5 µl of Hpy99III eluted from a heparin column.

Article Snippet: Extracts were incubated with bacteriophage λ DNA, phage T7 DNA, and adenovirus-2 DNA in NEBuffers 2 and 4 (New England Biolabs) at 37°C for 15 min and the digestion products were analyzed by agarose gel electrophoresis.

Techniques: Agarose Gel Electrophoresis, Electrophoresis

Journal: International Journal of Environmental Research and Public Health

Article Title: Application of an Ultrasonic Nebulizer Closet in the Disinfection of Textiles and Footwear

doi: 10.3390/ijerph191710472

Figure Lengend Snippet: Incubation conditions for bacteria cultivation in agar medium plates.

Article Snippet: B. atrophaeus DSM 2277, bacteriophage Lambda DSM 4499, E. coli DSM 4230 (bacteriophage Lambda host), E. coli DSM 30083, P. aeruginosa DSM 1117, S. aureus DSM 20231, and B. subtilis DSM 10 were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany.

Techniques: Incubation, Bacteria

Journal: International Journal of Environmental Research and Public Health

Article Title: Application of an Ultrasonic Nebulizer Closet in the Disinfection of Textiles and Footwear

doi: 10.3390/ijerph191710472

Figure Lengend Snippet: Log 10 reduction achieved in disinfection of a folded cotton towel of 460 × 720 mm by nebulization in a 0.08 m 3 closet. VIRCOV BAC 360 disinfectant was used at 1/3 ( v / v ) dilution. The indicators tested were B. atrophaeus DSM 2277 spores, bacteriophage Lambda DSM 4499, Escherichia coli DSM 30083, and Staphylococcus aureus DSM 20231. The disinfection time consisted of 2 min of disinfectant nebulization plus 5 min of rest without nebulization. The error bars in the graph represent the standard deviation of the mean of three replicates ( n = 3).

Article Snippet: B. atrophaeus DSM 2277, bacteriophage Lambda DSM 4499, E. coli DSM 4230 (bacteriophage Lambda host), E. coli DSM 30083, P. aeruginosa DSM 1117, S. aureus DSM 20231, and B. subtilis DSM 10 were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany.

Techniques: Standard Deviation

Journal: International Journal of Environmental Research and Public Health

Article Title: Application of an Ultrasonic Nebulizer Closet in the Disinfection of Textiles and Footwear

doi: 10.3390/ijerph191710472

Figure Lengend Snippet: Log 10 reduction achieved in disinfection of a footwear item by nebulization in a 0.08 m 3 closet. VIRCOV BAC 360 disinfectant was used at 1/3 ( v / v ) dilution. The indicators tested were B. atrophaeus DSM 2277 spores, bacteriophage Lambda DSM 4499, E. coli DSM 30083, and S. aureus DSM 20231. The disinfection time consisted of 2 min of disinfectant nebulization plus 5 min of rest without nebulization. The error bars in the graph represent the standard deviation of the mean of three replicates ( n = 3).

Article Snippet: B. atrophaeus DSM 2277, bacteriophage Lambda DSM 4499, E. coli DSM 4230 (bacteriophage Lambda host), E. coli DSM 30083, P. aeruginosa DSM 1117, S. aureus DSM 20231, and B. subtilis DSM 10 were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany.

Techniques: Standard Deviation

Journal: International Journal of Environmental Research and Public Health

Article Title: Application of an Ultrasonic Nebulizer Closet in the Disinfection of Textiles and Footwear

doi: 10.3390/ijerph191710472

Figure Lengend Snippet: Log 10 reduction achieved in disinfection of fabric by nebulization in the upscaled 0.58 m 3 closet. VIRCOV BAC 360 disinfectant was used at a 1/3 ( v / v ) dilution. The indicators tested were B. atrophaeus DSM 2277 spores, bacteriophage Lambda DSM 4499, E. coli DSM 30083, Pseudomonas aeruginosa DSM 1117, S. aureus DSM 20231, and Bacillus subtilis DSM 10. For the disinfection time, a short disinfection cycle and a long disinfection cycle were tested. The short disinfection cycle consisted of 2 min of nebulization, 2 min of rest without nebulization, and 2 min of aerosol extraction from the closet interior. For its part, the long disinfection cycle consisted of 4 min of nebulization, 4 min of rest without nebulization, and 2 min of aerosol extraction from the closet interior. The error bars in the graph represent the standard deviation of the mean of three replicates ( n = 3). Statistical difference between groups was evaluated by two-way ANOVA with post hoc comparisons made by the Tukey test. ns: not significant with p -value > 0.05; *: significant with p -value < 0.05; ***: significant with p -value < 0.001.

Article Snippet: B. atrophaeus DSM 2277, bacteriophage Lambda DSM 4499, E. coli DSM 4230 (bacteriophage Lambda host), E. coli DSM 30083, P. aeruginosa DSM 1117, S. aureus DSM 20231, and B. subtilis DSM 10 were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany.

Techniques: Aerosol, Extraction, Standard Deviation